Decoding epigenetic programs in differentiation and disease

Christina Leslie
Sloan Kettering Institute
11.16.2018 11:00 to 12:00
CSI 2117

Dysregulated epigenetic programs are a feature of many cancers, and the diverse differentiation states of immune cells as well as their dysfunctional states in tumors are in part epigenetically encoded. We will present recent analysis work and computational methodologies from our lab to decode epigenetic programs from genome-wide data sets.

We will first describe recent collaborative work to decipher chromatin states governing T cell dysfunction in cancer. Through analysis of the dynamics of chromatin accessibility using ATAC-seq and gene expression using RNA-seq in a mouse cancer model, we show that tumor-specific T cells differentiate to dysfunction through two discrete chromatin states: an initial plastic state that can be functionally rescued (i.e. through immunotherapy) and a later fixed state that is resistant to therapeutic reprogramming. We will also show how transcription factor (TF) motif analysis identifies potential drivers of global changes accessibility changes, and how in vivo pharmacological modulation of identified TFs decreases or delays progression to fixed dysfunction. Next, we will introduce new computational methodology to decipher transcriptional programs governing chromatin accessibility and gene expression in normal and dysfunctional T cell responses through a large-scale analysis of published data from mouse tumor and chronic viral infection models. This modeling shows that in all these systems — and contrary to current understanding of chronic infection — T cells commit to becoming dysfunctional early after an immune challenge, rather than first mounting and then losing an effector response. Finally, we will describe a novel machine learning approach called BindSpace to leverage massive in vitro TF binding data from SELEX-seq experiments through a joint embedding of DNA k-mers and TF labels, leading to improved prediction of TF binding.